A mitochondria-targeting ratiometric fluorescent probe for the detection of hypochlorite based on the FRET strategy

作者：Shen, Shi-Li^*; Zhang, Xiao-Fan; Ge, Yan-Qing; Zhu, Yan; Cao, Xiao-Qun^*

来源：RSC Advances, 2017, 7(87): 55296-55300.

DOI：10.1039/c7ra11086c

• TAP WATER ANALYSIS
• LIVING CELLS
• CANCER-CELLS
• SELECTIVE DETECTION
• REAL APPLICATION
• LIVE CELLS
• PH PROBE
• ACID
• HCLO
• MYELOPEROXIDASE

In this study, a ratiometric fluorescent probe (IRP) for -OCl was designed based on the fluorescence resonance energy transfer (FRET) platform. The probe was fabricated by integrating an imidazo[ 1,5-a] pyridine moiety (donor) with a rhodamine moiety (acceptor). We evaluated its properties for recognizing -OCl and the results implied that IRP possessed high selectivity, a real-time detection possibility and brilliant photostability. The fluorescence intensity ratios (I-575/I-467) of IRP displayed desirable -OCl dependent performance and responded linearly to -OCl in the concentration range of 0.5-3.5 mM. Also, IRP was applied to image endogenous -OCl with a mitochondria-targeting ability, due to the introduction of the quaternized pyridine moiety. Therefore, IRP may be used for further mitochondrial -OCl-related studies.

• 出版日期2017
• 单位山东第一医科大学