Antibiotic selectivity for prokaryotic vs. eukaryotic decoding sites

作者:Xie Yun; Dix Andrew V; Tor Yitzhak*
来源:Chemical Communications, 2010, 46(30): 5542-5544.
DOI:10.1039/c0cc00423e

摘要

A FRET assembly reports antibiotic affinities to two different RNA targets. A binder was labeled with a fluorophore that acts both as an acceptor for the emissive nucleoside on the bacterial A-site and a donor fluorophore for the terminally-labeled human A-site. Unlabeled drugs were used to dissociate the labeled antibiotic.

  • 出版日期2010