Down-regulation of interleukin-1 alpha-induced matrix metalloproteinase-13 expression via EP1 receptors by prostaglandin E-2 in human periodontal ligament cells

作者：Noguchi K^*; Ruwanpura SMPM; Yan M; Yoshida N; Ishikawa I

来源：Oral Microbiology and Immunology, 2005, 20(1): 56-59.

DOI：10.1111/j.1399-302X.2004.00187.x

• EP receptors
• interleukin-1 alpha
• matrix metalloproteinase-13
• periodontal ligament cells
• prostaglandin E2

In the present study, we investigated the effect of prostaglandin (PG) E-2 on matrix metalloproteinase (MMP)-13 production in human periodontal ligament cells stimulated with interleukin (IL)-1alpha. IL-1alpha enhanced both MMP-13 and PGE(2) production. Indomethacin, a nonselective cyclooxygenase inhibitor, and NS-398, a specific cyclooxygenase-2 (COX-2) inhibitor, significantly enhanced IL-1alpha-induced MMP-13 production in periodontal ligament cells, although both the agents completely inhibited IL-1alpha-induced PGE(2) production. Exogenous PGE(2) reduced IL-1alpha-induced MMP-13 mRNA and protein production in a dose-dependent manner. 17-phenyl-omega-trinor PGE(2), a selective EP1 receptor agonist, mimicked the inhibitory effect of PGE(2) on IL-1alpha-induced MMP-13 mRNA and protein production. On the basis of these data, we suggest that COX-2-dependent PGE(2) down-regulates IL-1alpha-elicited MMP-13 production via EP1 receptors in human periodontal ligament cells. PGE(2) may be involved in the regulation of destruction of extracellular matrix components in periodontal lesions.

• 出版日期2005-2
• 单位哈尔滨医科大学