AUTOMATED DNA SEQUENCING AND ANALYSIS OF 106 KILOBASES FROM HUMAN CHROMOSOME-19Q13.3

作者：MARTINGALLARDO A; MCCOMBIE WR; GOCAYNE JD; FITZGERALD MG; WALLACE S; LEE BMB; LAMERDIN J; TRAPP S; KELLEY JM; LIU LI; DUBNICK M; JOHNSTONDOW LA; KERL**AGE AR; DEJONG P; CARRANO A; FIELDS C; VENTER JC

来源：Nature Genetics, 1992, 1(1): 34-39.

DOI：10.1038/ng0492-34

A total of 116,118 basepairs (bp) derived from three cosmids spanning the ERCC1 locus of human chromosome 19q13.3 have been sequenced with automated fluorescence-based sequencers and analysed by polymerase chain reaction amplification and computer methods. The assembled sequence forms two contigs totalling 105,831 bp, which contain a human fosB proto-oncogene, a gene encoding a protein phosphatase, two genes of unknown function and the previously-characterized ERCC1 DNA repair gene. This light band region has a high average density of 1.4 Alu repeats per kilobase. Human chromosome light bands could therefore contain up to 75,000 genes and 1.5 million Alu repeats.

• 出版日期1992-4