Auxin and Cytokinin Affect Biomass and Bioactive Compound Production from Adventitious Roots of Eleutherococcus koreanum

作者：Lee Eun Jung; Kim Myong Ki; Paek Kee Yoeup^*

来源：Korean Journal of Horticultural Science and Technology, 2010, 28(4): 678-684.

• liquid culture
• medicinal plants
• root morphology

In an attempt to improve biomass and bioactive compound production, we cultured adventitious roots of Eleutherococcus koreanum in 250 mL Erlenmeyer flasks using Murashige and Skoog (MS) medium with different concentrations of auxins (IBA, NAA, IAA) and cytokinins (BA, kinetin, TDZ). Root biomass (fresh and dry weight) was enhanced at 5 mg center dot L(-1) indole-3-butyric acid (IBA) after 5 weeks of culture. The content of total phenolics and flavonoids was also increased with 5 mg center dot L(-1) IBA compared to alpha-naphtalene acetic acid (NAA) or indole-3-acetic acid (IAA) treatments. The combination of 5 mg center dot L(-1) IBA with 0.1 mg center dot L(-1) thidiazuron (TDZ; N-phenyl-N'-1,2,3,-thidiazol-5-ylurea) enhanced root fresh and dry weight (1.4- and 1.6-fold, respectively) as well as the content of total phenolics and flavonoids compared to the relative control (without cytokinin). On the contrary, N(6)-benzyladenine (BA) and 6-furfurylaminopurine (kinetin) did not significantly affect root biomass and bioactive compound production in adventitious roots of E. koreanum. These results suggested that 5 mg center dot L(-1) IBA combination with 0.1 mg center dot L(-1) TDZ supplementation was most suitable for both biomass and bioactive compound production from adventitious roots of E. koreanum.

• 出版日期2010-8