Determination of short-chain fatty acids in serum by hollow fiber supported liquid membrane extraction coupled with gas chromatography

作者：Zhao Guohua; Liu Jing fu; Nyman Margareta^*; Jonsson Jan Ake

来源：Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 2007, 846(1-2): 202-208.

DOI：10.1016/j.jchromb.2006.09.027

• hollow fiber supported liquid membrane extraction
• short-chain fatty acid (SCFA)
• serum
• gas chromatography
• determination

A method based on hollow fiber supported liquid membrane extraction coupled with a gas chromatograph equipped with flame ionization detector (GC-FID) was developed for the determination of six short-chain fatty acids including acetic acid, propionic acid, i-butyric acid, n-butyric acid, i-valeric acid and n-valeric acid in serum. Hollow fiber supported liquid membrane extraction was employed for preconcentration and clean-up of the samples. The fatty acids were extracted from the acidic donor (diluted serum) into a liquid membrane formed in the wall of the hollow fiber with 10% tri-n-octylphoshphine oxide (TOPO) in di-n-hexyl ether, and then extracted back into a basic acceptor solution filled in the lumen of the hollow fiber. After being acidified with HCI, the acceptor was directly analyzed by GC-FID. The acceptor concentration, donor pH, membrane liquid and extracting time were optimized giving an enrichment factor up to 155 times. The good linearity (r(2) > 0.980), reasonable recovery (87.2-121%), and satisfactory intro-assay (8.2-11.5%) and inter-assay (6.1-11.6%) precision illustrated the good performance of the present method. Limits of detection (LOD) ranged from 0.04 to 0.24 mu M and limits of quantification (LOQ) varied from 0.13 to 0.80 mu M.

• 出版日期2007-2-1
• 单位西南大学