A pharmacoproteomic study confirms the synergistic effect of chondroitin sulfate and glucosamine

作者：Calamia Valentina; Mateos Jesus; Fernandez Puente Patricia; Lourido Lucia; Rocha Beatriz; Fernandez Costa Carolina; Montell Eulalia; Verges Josep; Ruiz Romero Cristina; Blanco Francisco J^*

来源：Scientific Reports, 2014, 4(1): 5069.

DOI：10.1038/srep05069

Osteoarthritis (OA) is the most common age-related rheumatic disease. Chondrocytes play a primary role in mediating cartilage destruction and extracellular matrix (ECM) breakdown, which are main features of the OA joint. Quantitative proteomics technologies are demonstrating a very interesting power for studying the molecular effects of some drugs currently used to treat OA patients, such as chondroitin sulfate (CS) and glucosamine (GlcN). In this work, we employed the iTRAQ (isobaric tags for relative and absolute quantitation) technique to assess the effect of CS and GlcN, both alone and in combination, in modifying cartilage ECM metabolism by the analysis of OA chondrocytes secretome. 186 different proteins secreted by the treated OA chondrocytes were identified. 36 of them presented statistically significant differences (p %26lt;= 0.05) between untreated and treated samples: 32 were increased and 4 decreased. The synergistic chondroprotective effect of CS and GlcN, firstly reported by our group at the intracellular level, is now demonstrated also at the extracellular level.

• 出版日期2014-6-10