Non-toxic Salvia sclareoides Brot. extracts as a source of functional food ingredients: Phenolic profile, antioxidant activity and prion binding properties

作者：Rauter Amelia P^*; Dias Catarina; Martins Alice; Branco Isabel; Neng Nuno R; Nogueira Jose M; Goulart Margarida; Silva Filipa V M; Justino Jorge; Trevitt Clare; Waltho Jon P

来源：Food Chemistry, 2012, 132(4): 1930-1935.

DOI：10.1016/j.foodchem.2011.12.028

• Salvia sclareoides
• Phenolic profile
• Antioxidant activity
• Cytotoxicity
• Genotoxicity
• Prion binding

Salvia sclareoides is an aromatic herb native to Portugal. of which phenolic content (Folin-Ciocalteau method), chemical profile (HPLC/DAD), antioxidant activity (DPPH, beta-carotene/linoleic acid assays), acute toxicity (MTT method, adapted for non-adherent cells), genotoxicity (short-term chromosomal aberration assay) and prion binding properties were evaluated in the acetone, water, ethanol, methanol and n-butanol extracts. The latter presented the highest phenolic content and antioxidant activity (DPPH assay), and was the single one with the flavonoids (+)-catechin, kaempferol O-glucoside and quercetin. Vanillic acid was the major component of all extracts but gallic, gentisic, caffeic, syringic, coumaric and ferulic acids were also found in some extracts. Only the n-butanol extract had components binding to the cellular form of human prion protein detected by NMR which showed specificity for two regions of the folded domain and for the unstructured N-terminal region. Extracts were not cytotoxic nor genotoxic, reinforcing the potential of S. sclareoides for nutraceutical purposes.

• 出版日期2012-6-15