Developmental cigarette smoke exposure II: Kidney proteome profile alterations in 6 month old adult offspring

作者：Neal Rachel E^*; Jagadapillai Rekha; Chen Jing; Webb Cynthia L; Stocke Kendall; Gambrell Cailtin; Greene Robert M; Pisano M Michele

来源：Reproductive Toxicology, 2016, 65: 425-435.

DOI：10.1016/j.reprotox.2016.05.008

• Developmental cigarette smoke exposure
• CSE
• Proteome
• Kidney
• Mouse
• Inhalation
• Tobacco

Cigarette smoke exposure (CSE) during gestation and early development suppresses the growth trajectory in offspring. In prior studies utilizing a mouse model of 'active' developmental CSE (GD1-PD21), low birth weight induced by CSE persisted throughout the neonatal period and was present at the cessation of exposure at weaning with proportionally smaller kidney mass that was accompanied by impairment of carbohydrate metabolism. In the present study, littermates of those characterized in the prior study were maintained until 6 months of age at which time the impact of developmental CSE on the abundance of proteins associated with cellular metabolism in the kidney was examined. Kidney protein abundances were examined by 2D-SDS-PAGE based proteome profiling with statistical analysis by Partial Least Squares Discriminant Analysis. Key findings of this study include a persistence of impact of developmental CSE past the original exposure period on the nucleic acid and carbohydrate metabolism networks and oxidant scavenging pathways.

• 出版日期2016-10