Development of a sperm cryopreservation protocol for the mud spiny lobster, Panulirus polyphagus

作者：Fatihah S N; Jasmani S; Abol Munafi A B; Noorbaiduri S; Muhd Farouk H; Ikhwanuddin M^*

来源：Aquaculture, 2016, 462: 56-63.

DOI：10.1016/j.aquaculture.2016.04.025

• Cryoprotectant
• Extender
• Liquid nitrogen
• Cooling rates
• Thawing temperature
• Crustacean

This study aimed to develop a cryopreservation protocol for sperm of the mud spiny lobster, Panulirus polyphagus. Sperm of P. polyphagus were successfully cryopreserved using a protocol with cooling periods of 15 min per temperature, 25, 20, 16, 4, 2, -4, -20, -80, and -150 degrees C, followed by immediate storage in liquid nitrogen (at -196 degrees C). The efficacy of the cryopreserved protocol was determined by assessing the viability of sperm. The optimal thawing temperature for cryopreservation of sperm was 26 degrees C for 30 s, with a viability rate of 76.09% +/- 7.81. At room temperature, -20 and -80 degrees C, 10% glycine provided the highest percentage of sperm viability at 91.87 +/- 2.03% (5 min at room temperature), 91.31 +/- 2.65% (6 h at -20 degrees C) and 75.88 +/- 10.81% (6 h at - 80 degrees C). In conclusion, we developed a protocol (Protocol I) for the successful cryopreservation of P. polyphagus sperm using Ca-F saline as an extender and 10% glycine as a cryoprotectant.

• 出版日期2016-9-1