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摘要
Objective: We evaluated the value of high-throughput protein array in clinical screening for tumor markers. Method: HuProt array, which contained over 19000 proteins, was used. The case group consisted of 160 patients with various tumors and the control group consisted of 160 healthy subjects. The serum was collected and detected using protein array. The positive criteria were determined and the protein array data were interpreted according to the criteria by searching the databases and sequence alignment. The differences between the two groups were compared and the validity of protein array detection for serum tumor markers was confirmed. Results: The criteria were based on signal-to-noise ratio (SNR) combined visual inspection. This method had high sensitivity and specificity for interpreting protein array data. For the positive result based on SNR >= 10 and number of positive spots in visual inspection >= 6/20, the detection had 100% sensitivity and 100% specificity. The differences compared with other criteria reached significance level (P<0.05). For BLAST analysis, the amino acid sequences of 45 positive protein spots related to important tumor markers were selected. The protein array detection in the two groups indicated that the tumor markers in patients of the case group could be effectively detected; the detection results showed significant difference from the control group (P<0.05). Conclusion: Tumor markers were accurately detected using HuProt array with the criteria of SNR >= 10 and number of positive spots in visual inspection >= 6/20. The high-throughput protein array was clinically valuable in tumor screening.
- 出版日期2016
- 单位武汉大学
