A sensitive, specific and reproducible real-time polymerase chain reaction method for detection of Plasmodium vivax and Plasmodium falciparum infection in field-collected anophelines

作者：Bickersmith Sara A^*; Lainhart William; Moreno Marta; Chu Virginia M; Vinetz Joseph M; Conn Jan E

来源：Memorias do Instituto Oswaldo Cruz, 2015, 110(4): 573-576.

DOI：10.1590/0074-02760150031

• Anopheles
• Plasmodium
• TaqMan
• real-time PCR

We describe a simple method for detection of Plasmodium vivax and Plasmodium falciparum infection in anophelines using a triplex TaqMan real-time polymerase chain reaction (PCR) assay (18S rRNA). We tested the assay on Anopheles darlingi and Anopheles stephensi colony mosquitoes fed with Plasmodium-infected blood meals and in duplicate on field collected An. darlingi. We compared the real-time PCR results of colony-infected and field collected An. darlingi, separately, to a conventional PCR method. We determined that a cytochrome b-PCR method was only 3.33% as sensitive and 93.38% as specific as our real-time PCR assay with field-collected samples. We demonstrate that this assay is sensitive, specific and reproducible.

• 出版日期2015-6