A site-specific branching poly-glutamate tag mediates intracellular protein delivery by cationic lipids

作者：Huang, Si-Qi; Han, Bei-Bei; Li, Yan-Mei; Chen, Yong-Xiang^*

来源：Biochemical and Biophysical Research Communications, 2018, 503(2): 671-676.

DOI：10.1016/j.bbrc.2018.06.059

• Intracellular protein delivery
• Protein modification
• Negatively charged peptide tag
• Cationic lipid

Intracellular protein delivery is of significance for cellular protein analysis and therapeutic development, but remains challenging technically. Herein, we report a general and highly potent strategy for intracellular protein delivery based on commercially available cationic lipids. In this strategy, a designed double branching poly-glutamate tag is site-specifically attached onto the C-terminal of protein cargos via expressed protein ligation (EPL), which mediates the entrapment of proteins into cationic liposomes driven by electrostatic interaction. The resultant protein-lipid complexes can enter into cytosol with a high efficiency even at the low protein concentration while maintaining protein's biological activity.

• 出版日期2018-9-5
• 单位清华大学; 首都医科大学