Nucleation of hydroxyapatite crystals by self-assembled Bombyx mori silk fibroin

作者：Yang, Mingying^*; He, Wen; Shuai, Yajun; Min, Sijia; Zhu, Liangjun

来源：Journal of Polymer Science Part B: Polymer Physics , 2013, 51(9): 742-748.

DOI：10.1002/polb.23249

• B. mori silk fibroin
• biomineralization
• biopolymers
• hydroxyapatite crystals
• interfaces
• nucleation
• self-assembly

Bombyx mori silk fibroin (SF) is known to be capable of facilitating nucleation of the hydroxyapatite crystals (HAps). To find out how SF mediates the nucleation of HAps, self-assembly of SF in 1.5 simulated body fluid (SBF) was observed in this study through design of a co-solution of SF and 1.5-times SBF (SF/1.5 SBF). After the co-solution of SF/1.5 SBF was incubated at 37.2 degrees C up to 7 days, SEM, X-ray, and Fourier transform infrared (FTIR) observations indicated that nucleation of HAps was increased. In addition, the structure of SF was transited from random coil into -sheet indicated by FTIR spectra. The -sheet assembly of SF in 1.5 SBF was also supported by CD spectra. Atomic force microscopy provided detailed progress of the self-assembly that SF incubated in 1.5 SBF was self-assembled in the form from dot, through rod to final net. Therefore, this study suggested that nucleation of HAps of SF was controlled by its molecular self-assembly.

• 出版日期2013-5-1
• 单位浙江大学