Direct next-generation sequencing of virus-human mixed samples without pretreatment is favorable to recover virus genome

作者：Li, Dingchen; Li, Zongwei; Zhou, Zhe; Li, Zhen; Qu, Xinyan; Xu, Peisong; Zhou, Pingkun; Bo, Xiaochen^*; Ni, Ming

来源：Biology Direct, 2016, 11(1): 3.

DOI：10.1186/s13062-016-0105-x

• Pathogen genome recovery
• Nonculture
• Background Depletion
• Whole Transcriptome Amplification
• Next-generation sequencing

Next-generation sequencing (NGS) enables the recovery of pathogen genomes from clinical samples without the need for culturing. Depletion of host/microbiota components (e.g., ribosomal RNA and poly-A RNA) and whole DNA/cDNA amplification are routine methods to improve recovery results. Using mixtures of human and influenza A virus (H1N1) RNA as a model, we found that background depletion and whole transcriptome amplification introduced biased distributions of read coverage over the H1N1 genome, thereby hampering genome assembly. Influenza serotyping was also affected by pretreatments. We propose that direct sequencing of noncultured samples without pretreatment is a favorable option for pathogen genome recovery applications.

• 出版日期2016-1-12
• 单位中国人民解放军军事医学科学院; 北京中医药大学