Nitric oxide promotes survival of cerebellar granule neurons cultured in vitro through the Akt pathway

作者：Wang, Lin; Li, Mei; Zhou, Lihua^*

来源：Neural Regeneration Research, 2011, 6(20): 1559-1563.

DOI：10.3969/j.issn.1673-5374.2011.20.007

• nitric oxide
• cerebellar granule neurons
• development
• apoptosis
• Akt
• cyclic guanosine monophosphate
• neural regeneration

In this study, cerebellar granule neurons were used to examine the role of nitric oxide on cell survival. The N-methyl-D-aspartic acid receptor antagonist, MK-801, and the soluble guanylate cyclase antagonist, 1H-[1, 2, 4] oxadiazolo-[4, 3-a] quinoxalin-1-one, decreased cell viability, induced caspase-3, and decreased phosphorylated-Akt levels, suggesting that blockade of nitric oxide production promotes apoptosis of differentiating cerebellar granule neurons. After administration of sodium nitroprusside, an endogenous nitric oxide donor, cell viability recovered, caspase-3 expression was decreased, and phosphorylated-Akt levels increased. This study provides direct evidence that nitric oxide can sustain the survival of developing cerebellar granule neurons in vitro through the nitric oxide-Akt pathway. Moreover, endogenous nitric oxide exerts these effects in a cyclic guanosine monophosphate-dependent manner while exogenous nitric oxide does so in a cyclic guanosine monophosphate-independent manner.

• 出版日期2011-7
• 单位中山大学