科研之友
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摘要
The antioxidant and anti inflammatory activities of the methanol: chloroform (1:1) extracts of 16 Salvia species indigenous to South Africa were evaluated. Antioxidant activity was measured using the 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and the 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging assays and compared to the control values obtained with Trolox (R). Nearly all the solvent extracts displayed antioxidant activity, with the IC(50) value ranging from 1.6 to 74.5 mu g/ml using DPPH(center dot), whilst the IC(50) values ranged from 11.9 to 69.3 mu g/ml, when tested with ABTS(center dot+). The extract of Salvia schlechteri, with an IC(50) value of 1.6 mu g/ml, was three times more active than the reference compound, Trolox (R) (IC(50) value: 2.51 mu g/ml). The antiinflammatory activity was evaluated using the 5-lipoxygenase assay. With the exception of Salvia radula (IC(50) value: 78.8 mu g/ml), the extracts displayed poor inhibition of the 5-lipoxygenase enzyme, with all IC(50) values being greater than 100 mu g/ml. The total phenolic content based on gallic acid equivalents (GAE) confirmed the presence of total soluble phenolics in the various extracts from 45 to 211 mg of GAE per g dry sample and showed strong association (r(2) = 0.90) with antioxidant activity. High-performance liquid chromatography (HPLC) was used to identify various compounds in the extracts. Betulafolientriol oxide and rosmarinic acid were detected in all the species investigated, and rosmarinic acid, carnosic acid, carnosol and oleanolic acid/ursolic acid were abundant in many species.
- 出版日期2010-3-15
