Phase behavior of mixtures of human lens proteins Gamma D and Beta B1

作者：Wang Ying; Lomakin Aleksey; McManus Jennifer J; Ogun Olutayo; Benedek George B^*

来源：Proceedings of the National Academy of Sciences, 2010, 107(30): 13282-13287.

DOI：10.1073/pnas.1008353107

• cataract
• crystallin
• phase separation
• mixture

We have experimentally determined the coexistence surface characterizing the phase behavior of gamma D-beta B1-water ternary solutions. The coexistence surface fully describes the solution conditions, i.e., temperature, protein concentration, and protein composition, at which liquid-liquid phase separation occurs in a ternary solution. We have observed a significant demixing of gamma D and beta B1 i.e., large difference of composition in the two coexisting phases. This demixing suggests that the energy of the gamma D-beta B1 attractive interaction is significantly smaller than the energy of the gamma D-gamma D attractive interaction. We also observed the lowering of the phase separation temperature upon increasing of the fraction of beta B1 in solution. We provide a theoretical analysis of our experimental data, which enables a quantitative description of our principal experimental findings. In this way, we have evaluated the magnitude and temperature dependence of the relevant interprotein interaction energies. Our findings provide insight into the factors essential for maintaining lens proteins in a single homogeneous phase, thereby enabling lens transparency.

• 出版日期2010-7-27