A novel expression system for production of soluble prion proteins in E. coli

作者:Abskharon Romany N N; Ramboarina Stephanie; El Hassan Hassan; Gad Wael; Apostol Marcin I; Giachin Gabriele; Legname Giuseppe; Steyaert Jan; Messens Joris; Soror Sameh H*; Wohlkonig Alexandre
来源:Microbial Cell Factories, 2012, 11: 6.
DOI:10.1186/1475-2859-11-6

摘要

Expression of eukaryotic proteins in Escherichia coli is challenging, especially when they contain disulfide bonds. Since the discovery of the prion protein (PrP) and its role in transmissible spongiform encephalopathies, the need to obtain large quantities of the recombinant protein for research purposes has been essential. Currently, production of recombinant PrP is achieved by refolding protocols. Here, we show that the co-expression of two different PrP with the human Quiescin Sulfhydryl OXidase (QSOX), a human chaperone with thiol/disulfide oxidase activity, in the cytoplasm of E. coli produces soluble recombinant PrP. The structural integrity of the soluble PrP has been confirmed by nuclear magnetic resonance spectroscopy, demonstrating that properly folded PrP can be easily expressed in bacteria. Furthermore, the soluble recombinant PrP produced with this method can be used for functional and structural studies.

  • 出版日期2012-1-10