Engineering of Self-Assembled Fibronectin Matrix Protein and Its Effects on Mesenchymal Stem Cells

作者：Yun Ye Rang; Pham Le B Hang; Yoo Yie Ri; Lee Sujin; Kim Hae Won^*; Jang Jun Hyeog

来源：International Journal of Molecular Sciences, 2015, 16(8): 19645-19656.

DOI：10.3390/ijms160819645

• fibronectin
• peptide amphiphile
• rat mesenchymal stem cells
• cellular activity

Fibronectin (FN) contributes to cell adhesion, proliferation, and differentiation in various cell types. To enhance the activity of fibronectin at the sites of focal adhesion, we engineered a novel recombinant fibronectin (FNIII10) fragment connected to the peptide amphiphile sequence (PA), LLLLLLCCCGGDS. In this study, the effects of FNIII10-PA on rat mesenchymal stem cells (rMSCs) were compared with those of FNIII10. FNIII10-PA showed the prominent protein adhesion activity. In addition, FNIII10-PA showed a significantly higher effect on adhesion, proliferation, and differentiation of rMSCs than FNIII10. Taken together, the FNIII10-containing self-assembled sequence enhanced rMSCs adhesion, proliferation, and differentiation.

• 出版日期2015-8