Aflatoxins and ochratoxin A in different cocoa clones (Theobroma cacao L.) developed in the southern region of Bahia, Brazil

作者：Maciel Leonardo Fonseca^*; de Souza Madureira Felicio Ana Lucia; Rodrigues Miranda Lucas Caldeirao; Pires Tassia Cavalcante; Bispo Eliete da Silva; Hirooka Elisa Yoko

来源：Food Additives and Contaminants Part A-Chemistry Analysis Control Exposure& Risk Assessment, 2018, 35(1): 134-143.

DOI：10.1080/19440049.2017.1397293

• Mycotoxins
• fluorescence
• co-occurrence

Brazil is the sixth largest producer of cocoa beans in the world, after Cote d'Ivoire, Ghana, Indonesia, Nigeria and Cameroon. The southern region of Bahia stands out as the country's largest producer, accounting for approximately 60% of production. Due to damage caused by infestation of the cocoa crop with the fungus Moniliophthora perniciosa, which causes witch's broom disease', research in cocoa beans has led to the cloning of species that are resistant to the disease; however, there is little information about the development of other fungal genera in these clones, such as Aspergillus, which do not represent a phytopathogenicity problem but can grow during the pre-processing of cocoa beans and produce mycotoxins. Thus, the aim of this work was to determine the presence of aflatoxin (AF) and ochratoxin A (OTA) in cocoa clones developed in Brazil. Aflatoxin and ochratoxin A contamination were determined in 130 samples from 13 cocoa clones grown in the south of Bahia by ultra-performance liquid chromatography with a fluorescence detector. The method was evaluated for limit of detection (LOD) (0.05-0.90 g kg(-1)), limit of quantification (0.10-2.50 g kg(-1)) and recovery (RSD) (89.40-95.80%) for AFB(1), AFB(2), AFG(1), AFG(2) and OTA. Aflatoxin contamination was detected in 38% of the samples in the range of

• 出版日期2018