A newly isolated Paecilomyces sp WSH-L07 for laccase production: isolation, identification, and production enhancement by complex inducement

作者：Liu Zhiyu; Zhang Dongxu; Hua Zhaozhe; Li Jianghua; Du Guocheng^*; Chen Jian

来源：Journal of Industrial Microbiology and Biotechnology, 2009, 36(10): 1315-1321.

DOI：10.1007/s10295-009-0615-y

• Laccase
• Screening
• Paecilomyces sp.
• Complex inducers

Laccase can catalyze the oxidation of a wide range of organic and inorganic substrates. In this study, an easily detectable method was employed for screening laccase-producing microorganisms by using 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) as laccase-secretion indicator. A novel laccase-producing strain was isolated and identified as Paecilomyces sp. WSH-L07 according to the morphological characteristics and the comparison of internal transcribed spacer (ITS) ribosomal DNA (rDNA) gene sequences. In further investigation, the production of laccase by Paecilomyces sp. WSH-L07 was greatly enhanced by the nontoxic inducers of copper sulphate and methylene blue. Under the induction of 50 A mu M copper sulphate and 20 A mu M methylene blue, the maximum laccase production was obtained. When these inducers were added into cultivation medium at 24 h and 12 h, respectively, an increment of about 100 times of laccase activity compared with that of in inducer-free medium and about two times of that of in single copper-supplemented medium was observed. Compared with other Paecilomyces species, Paecilomyces sp. WSH-L07 exhibit the better laccase-producing characteristics with an activity of 1,650 U/l on the eighth day, suggesting its potential ability for industrial application.

• 出版日期2009-10
• 单位江南大学