Antinociceptive and anti-inflammatory activities of a sulfated polysaccharide isolated from the green seaweed Caulerpa cupressoides

作者：Rodrigues Jose A G; Vanderlei Edfranck de S O; Silva Luana M C M; de Araujo Ianna W F; de Queiroz Ismael N L; de Paula Gabriela A; Abreu Ticiana M; Ribeiro Natassia A; Bezerra Mirna M; Chaves Helliada V; Lima Vilma; Jorge Roberta J B; Monteiro Helena S A; Leite Edda L; Benevides Norma M B^*

来源：Pharmacological Reports, 2012, 64(2): 282-292.

DOI：10.1016/s1734-1140(12)70766-1

• marine alga
• Caulerpa cupressoides
• sulfated polysaccharide
• nociception
• inflammation

Background: Red and brown algae sulfated polysaccharides (SPs) have been widely investigated as antinociceptive and/or anti-inflammatory agents; however, no description of these biological properties concerning green algae SPs have been reported. Caulerpa curpressoides (Chlorophyta) presents three SPs fractions (Cc-SP1, Cc-SP2, and Cc-SP3). Anticoagulant (in vitro) and anti- and pro-thrombotic (in vivo) effects of Cc-SP2 had been recently reported. We evaluated the effects of Cc-SP2 using models of nociception and acute inflammation in vivo.
Methods: Male Swiss mice received Cc-SP2 (iv) 30 min prior to receiving 0.6% acetic acid (10 ml/kg, ip), 1% formalin (20 mu l, sc) or were subjected to thermal stimuli (51 +/- 1 degrees C). Cc-SP2 was injected sc to male Wistar rats in a peritonitis model or a paw edema model using carrageenan (ip or ipl, 500 mu g). To analyze the systemic effects, Cc-SP2 (27 mg/kg, sc) was administrated to both genders mice before waiting for 14 days.
Results: Cc-SP2 (3, 9 or 27 mg/kg) reduced (p < 0.05) the number of writhes induced by acetic acid by 57, 89.9 and 90.6%, respectively, the licking time in the first (9 or 27 mg/kg with 42.47 and 52.1%, respectively) and the second (3, 9 or 27 mg/kg with 68.95, 82.34 and 84.61%, respectively) phases. In the hot-plate test, the antinociceptive effect of Cc-SP2 (9 mg/kg) was primarily observed at 60 min (26.7 +/- 1.2 s), with its effect reversed by naloxone (8.6 +/- 1.3 s), suggesting the involvement of the opioid system. Cc-SP2 (3, 9 or 27 mg/kg, sc, p < 0.05) showed anti-inflammatory effects by decreasing neutrophils migration by 64, 69 and 73%, respectively, and potently reduced the paw edema, especially at the second (0.16 +/- 0.02, 0.16 +/- 0.03 and 0.12 +/- 0.05 ml) and third (0.16 +/- 0.03, 0.18 +/- 0.02 and 0.14 +/- 0.04 ml) hours, respectively. Cc-SP2 did not cause hepatic or renal alterations or affect body mass or the macroscopy of the organs examined (p > 0.05). Histopathological analyses of the liver and kidney showed that both organs were affected by Cc-SP2 treatment, but these effects were considered reversible.
Conclusion: The results indicate that the analgesic and anti-inflammatory effects of Cc-SP2 could be of biomedical applicability as a new, natural tool in pain and acute inflammatory conditions.

• 出版日期2012-4