Modifying alginate with early embryonic extracellular matrix, laminin, and hyaluronic acid for adipose tissue engineering

作者：Chen Yo Shen; Chen Yen Yu; Hsueh Yu Sheng; Tai Hao Chih; Lin Feng Huei^*

来源：Journal of Biomedical Materials Research Part A, 2016, 104(3): 669-677.

DOI：10.1002/jbm.a.35606

• HA-L-Alg beads
• embryonic
• extracellular matrix
• adipose tissue engineering

Extracellular matrix provides both mechanistic and chemical cues that can influence cellular behaviors such as adhesion, migration, proliferation, and differentiation. In this study, a new material, HA-L-Alg, was synthesized by linking developmentally essential ECM constituents hyaluronic acid (HA) and laminin(L) to alginate (Alg). The fabrication of HA-L-Alg was confirmed by FTIR spectroscopy, and it was used to form 3D cell-carrying beads. HA-L-Alg beads had a steady rate of degradation and retained 63.25% of mass after 9 weeks. HA-L-Alg beads showed biocompatibility comparable to beads formed by Alg-only with no obvious cytotoxic effect on the embedded 3T3-L1 preadipocytes. HA-L-Alg encapsulated 3T3-L1 cells were found to have a higher proliferation rate over those in Alg-only beads. These cells also showed better differentiation capacity after 2 weeks of adipogenic induction within HA-L-Alg beads. These results support that HA-L-Alg facilitated cell survival and proliferation, as well as stimulated and maintained cell differentiation. Our results suggest that HA-L-Alg has a great clinical potential to be used as stem cell carrier for adipose tissue engineering.

• 出版日期2016-3