Differential expression of matrix metalloproteinases and their tissue inhibitors in human primary cultured prostatic cells and malignant prostate cell lines

作者：Zhang J; Jung K^*; Lein M; Kristiansen G; Rudolph B; Hauptmann S; Schnorr D; Loening SA; Lichtinghagen R

来源：Prostate, 2002, 50(1): 38-45.

DOI：10.1002/pros.10030

• prostate cancer
• epithelial cells
• stromal cells
• reverse transcriptional polymerase chain reaction

BACKGROUND. The aim was to investigate the expression of matrix metalloproteinases (MMPs), membrane type MMPs (MT-MMPs), and their inhibitors (TIMPs) in human primary cultured prostatic cells and malignant prostate cell lines.
METHODS. Reverse transscription-polymerase chain reaction-based measurements of the mRNA levels of MMP-2, MMP-7, MT1-MMP, MT3-MMP, TIMP-1, and TIMP-2 in relation to the house-keeping gone glyceraldehyde phosphate dehydrogenase were performed in cancerous and non-cancerous prostatic tissue samples, in primary cell cultures of epithelial cells, in both fibroblasts, and smooth-muscle cells as stromal cells, and in the human malignant prostatic cell lines DU-145, LNCaP, and PC-3.
RESULTS. MMP-2 was mainly expressed in the stromal cells and MMP-7 showed their highest values in the epithelial cells. MTI-MMP, MT3-MMP, TIMP-1, and TIMP-2 were found both in the stromal and in the epithelial cells, but there were sonic differences between the expressions in fibroblasts and smooth-muscle cells. Different expressions were also observed between the cells deriving from the primary cell cultures, the benign cell line BPH-1, and the malignant cell lines LNCaP, D-145, and PC-3.
CONCLUSIONS. These exemplary results concerning different expressions of MMPS and TIMPs in cells from prostatic tissue suggest that a better insight into changes observed in prostatic tissue needs studies on cells cultured from the tissue.

• 出版日期2002-1-1
• 单位南开大学