Anticancer activities of two sesquiterpene lactones, millerenolide and thieleanin isolated from Viguiera sylvatica and Decachaeta thieleana

作者：Taylor Peter G^*; Loo Omar A Dupuy; Bonilla Jose A; Murillo Renato

来源：Fitoterapia, 2008, 79(6): 428-432.

DOI：10.1016/j.fitote.2007.07.019

• Viguiera sylvatica
• Decachaeta thieleana
• B16/BL6 melanoma
• millerenolide
• thieleanin

The present study was carried out in order to examine the anticancer properties of two sesquiterpene lactones, millerenolide and thieleanin, isolated from Viguiera sylvatica and Decachaeta thieleana, against cell lines in vitro, and on the growth B16/BL6 melanoma tumors in C57BL/6 mice. Millerenolide and thieleanin showed a similar pattern of cytotoxicity with the greatest effect on viability being evident with A549 human lung cancer cells (IC50 - 40 and 32 mu M respectively), and with the 3T3/HER2 cell line which are 3T3 mouse fibroblasts transfected with the HER2 oncogene (IC50 - 16 and 28 mu M respectively). The parent 3T3 cells and the B16/BL6 mouse melanoma cells were less sensitive to these compounds, with thieleanin showing an IC50 with B16/BL6 greater than the highest dose tested (203 mu M). Treatment with millerenolide (8 mg/kg, i.p. on days 0, 2 and 4 post-inoculation) significantly inhibited the growth of subcutaneous B16/BL6 tumors in C57BL/6 mice, (50% inhibition at day 25, P=0.015), as well as retarding the appearance of detectable tumor (millerenolide - day 15.2 +/- 0.4 vs control - day 12.8 +/- 0.5, mean +/- SEM, P = 0.011). In contrast, treatment with thieleanin (8 mg/kg every other day up to the day of kill) neither retarded the appearance of the tumor nor its growth.

• 出版日期2008-9