Microarray-based identification of Pitx3 targets during Xenopus embryogenesis

作者：Hooker Lara; Smoczer Cristine; KhosrowShahian Farhad; Wolanski Marian; Crawford Michael J^*

来源：Developmental Dynamics, 2012, 241(9): 1487-1505.

DOI：10.1002/dvdy.23836

• microarray
• Pitx3
• morpholino
• Xenopus laevis
• eye
• lens
• somite
• Pax6
• segmentation clock
• retinoid
• mutant

Background: Unexpected phenotypes resulting from morpholino-mediated translational knockdown of Pitx3 in Xenopus laevis required further investigation regarding the genetic networks in which the gene might play a role. Microarray analysis was, therefore, used to assess global transcriptional changes downstream of Pitx3. Results: From the large data set generated, selected candidate genes were confirmed by reverse transcriptase-polymerase chain reaction (RT-PCR) and in situ hybridization. Conclusions: We have identified four genes as likely direct targets of Pitx3 action: Pax6, beta Crystallin-b1 (Crybb1), Hes7.1, and Hes4. Four others show equivocal promise worthy of consideration: Vent2, and Ripply2 (aka Ledgerline or Stripy), eFGF and RXRa. We also describe the expression pattern of additional and novel genes that are Pitx3-sensitive but that are unlikely to be direct targets. Developmental Dynamics 241:14871505, 2012.

• 出版日期2012-9