Non-Covalent Albumin Conjugates of Silicon(IV) Phthalocyanines Axially Substituted with Nucleoside: Preparation and in vitro Photodynamic Activities

作者：Yang Xiao-mei; Zheng Bi-yuan; Cai Yue; Lin Ai-lan; Shen Xiao-min; Zhang Han-hui; Huang Jian-dong^*

来源：Spectroscopy and Spectral Analysis, 2015, 35(2): 457-461.

DOI：10.3964/j.issn.1000-0593(2015)02-0457-05

• Silicon phthalocyanine
• Albumin
• Non-covalent conjugate
• Photodynamic activity
• Nucleoside

The interactions of bovine serum albumin (BSA) with five novel silicon (IV) phthalocyanines(SiPc1-5) axially modified by nucleosides (cytidine, 5-N-cytidine, methyl cytidine, uridine and methyl uridine) derivatives were studied by fluorescence spectroscopy. The results show that there are strong interactions between these silicon phthalocyanines and BSA with a binding constant of(4. 90 similar to 83. 18) x10(5) mol(-1) . L. Therefore, the non-covalent BSA conjugate of bis(2',3'-O-isopropyl-cytidine-oxy) phthalocyaninatosilicon(IV) (SiPc1) was further been prepared. The molar ratio of phthalocyanine to albumin was found to be 1 : 1 for the obtained SiPc1-BSA conjugate. The absorption spectra of SiPc1 and SiPc1-BSA in the visible region have no significant difference, both showing an Q-band maximum at about 686 inn. It indicates that the spectroscopic characteristics of SiPc1 are not affected by binding to albumin. The SiPc1-BSA conjugate exhibits high photodynamic activity against human hepatoma cell line HepG2 with an IC50 value of 3. 0x10(-7) mol . L-1. By comparsion, SiPc1-BSA has a higher photodynamic activity than SiPc1 (in PBS formation, IC50=7. 0x10(-7) mol . L-1), which can be attributed to its higher cellular uptake.

• 出版日期2015-2
• 单位福州大学