Aflatoxins and ochratoxin A in maize of Punjab, Pakistan

作者：Iram Wajiha; Anjum Tehmina^*; Abbas Mateen; Khan Abdul Muqeet

来源：Food Additives & Contaminants Part B-Surveillance, 2014, 7(1): 57-62.

DOI：10.1080/19393210.2013.843205

• aflatoxins
• moisture
• maize
• HPLC
• immunoaffinity

Aflatoxin and ochratoxin levels were determined in maize samples collected from store houses of 15 districts belonging to three agro-ecological zones of Punjab, Pakistan. Toxins were extracted by Aflaochra immunoaffinity columns and analysed by high-performance liquid chromatography (HPLC). Mean moisture content of maize kernels was recorded above the safe storage level of 15%. Results indicated that aflatoxin B1 and B2 contamination was found in 97.3% and 78.9% of the collected samples, respectively. Aflatoxin G1, aflatoxin G2 and ochratoxin A were not detected in any sample. Among positive samples, 77.3% contained aflatoxin B1 and 28% aflatoxin B2, exceeding the legal limits as set by the European Union (EU) and the United States Food and Drug Administration (USFDA). It was concluded that a significant number of samples contained aflatoxin B1 and B2 above the legal limits.

• 出版日期2014-1-2