科研之友
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摘要
Biological tissues possessing motional heterogeneity are difficult to comprehend at atomic level in their native state. Some of these tissues play crucial roles in support and movement of the body. Articular cartilage is one such tissue exemplified with highly mobile glycosaminoglycans (GAGS, correlation time similar to 10(-9)s) and relatively rigid collagen protein (correlation time similar to 10(-6) s). Simultaneous study of both the components at atomic level is challenging and requires different sets of solid state nuclear magnetic resonance (ssNMR) experiments for mobile and rigid parts. Additionally, it is hard to get any quantitative information as one C-13 one pulse experiment is time-consuming. We present here a C-13 ssNMR experiment with complete molecular characterization of cartilage organic components in its native state. We achieved this by employing heteronuclear Overhauser enhancement with equilibration of magnetization by low power irradiation. Complete C-13 resonances from GAGS and collagen were identified simultaneously with 2-6 times sensitivity enhancement. We are also providing a method to characterize GAGs (qualitatively and quantitatively) in native state thereby increasing its applicability in disease conditions where GAGS is degraded. This method is not only applicable to biological tissues such as,cartilage but also to understand, ultrastructural details of hydrogels and other motional heterogeneous biomaterials used in cartilage tissue engineering.
- 出版日期2016-9-29
