Adipose stromal cells improve healing of vocal fold scar: Morphological and functional evidences

作者：de Bonnecaze Guillaume^*; Chaput Benoit; Woisard Virginie; Uro Coste Emmanuelle; Swider Pascal; Vergez Sebastien; Serrano Elie; Casteilla Louis; Planat Benard Valerie

来源：Laryngoscope, 2016, 126(8): E278-E285.

DOI：10.1002/lary.25867

• Wound healing
• cytotherapy
• vocal fold scar
• adipose stromal cells
• biomechanical analysis

Objectives/HypothesisAdipose derived stromal cells (ASCs) are abundant and easy to prepare. Such cells may be useful for treating severe vocal disturbance caused by acute vocal fold scars.
Study DesignProspective animal experiments with controls.
MethodsTwenty New-Zealand white rabbits were used in the present study. We evaluated vocal fold healing, with or without injection of autologous ASCs, after acute scarring. A defined lesion was created and the ASCs were immediately injected. Vocal fold regeneration was evaluated histomorphometrically and via viscoelastic analysis using an electrodynamic shaker.
ResultsSix weeks after ASC injection, vocal folds exhibited significantly less inflammation than control folds (P < 0.005). In addition, hypertrophy of the lamina propria and fibrosis were significantly reduced upon ASC injection (P < 0.02). The decrease in viscoelastic parameters was less important in the ASC injected group compared to the noninjected group (P = 0.08).
ConclusionInjection of autologous ASCs improved vocal fold healing in our preclinical model. Further studies are needed, but this method may be useful in humans.

• 出版日期2016-8