Facile synthesis of hydroxymethylcytosine-containing oligonucleotides and their reactivity upon osmium oxidation

作者：Sugizaki Kaori; Ikeda Shuji; Yanagisawa Hiroyuki; Okamoto Akimitsu^*

来源：Organic and Biomolecular Chemistry, 2011, 9(11): 4176-4181.

DOI：10.1039/c1ob05247k

DNA strands containing a 5-hydroxymethylcytosine ((hm)C), which have recently been found in neuron cells and embryonic stem cells, were synthesized through a facile synthetic technique. The (hm)C-containing strands were efficiently oxidized at (hm)C using an osmium oxidation assay. The (hm)C was oxidized as easily as 5-methylcytosine, which can be distinguished from unmethylated cytosine.

• 出版日期2011