A new fluorescent oligonucleotide probe for in situ detection of s-triazine-degrading Rhodococcus wratislaviensis in contaminated groundwater and soil samples

作者：Grenni Paola; Gibello Alicia; Caracciolo Anna Barra^*; Fajardo Carmen; Nande Mar; Vargas Raquel; Sacca Maria Ludovica; Jose Martinez Inigo Maria; Ciccoli Roberto; Martin Margarita

来源：Water Research, 2009, 43(12): 2999-3008.

DOI：10.1016/j.watres.2009.04.022

• Terbuthylazine
• Biodegradation
• Groundwater
• FISH
• Probe

A bacterial strain (FPA1) capable of using terbuthylazine, simazine, atrazine, 2-hydroxysimazine, deethylatrazine, isopropylamine or ethylamine as its sole carbon source was isolated from a shallow aquifer chronically contaminated with s-triazine herbicides. Based on its 16S rDNA sequence analysis, the strain FPA1 was identified as Rhodococcus wratislaviensis. The disappearance time of 50% of the initial terbuthylazine concentration in the presence of this strain (DT50) was 62 days. This strain was also able to mineralise the [U-ring C-14] triazine-ring, albeit at a slow rate. A 16S rRNA target oligonucleotide probe (RhLu) was designed, and the FISH protocol was optimised, in order to detect R. wratisloviensis in striazine-contaminated sites. The RhLu probe gave a positive signal (expressed as % of total DAPI-positive cells) in both the groundwater (2.19 +/- 0.41%) and soil (2.10 +/- 0.96%) samples analysed. Using the RhLu probe, R. wratislaviensis can be readily detected, and its population dynamics can be easily monitored, in soil and in water ecosystems contaminated with s-triazine. To the best of our knowledge, this is the first report showing the isolation, from groundwater, of a bacterial strain able to degrade s-triazines.

• 出版日期2009-7