Differentiation of Isomeric Polyphenolic Glycosides That Possess Regioisomeric Acylated Monosaccharide Residues by Electrospray Ionization-Tandem Mass Spectrometry

作者：Liao Shang Gao^*; Wang Zhen; Sun Fa; Zhang Li Juan; He Xun; Zheng Lin; Wang Ai Min; Li Yong Jun; Huang Yong; Lan Yan Yu; Wang Yong Lin

来源：Spectroscopy Letters, 2014, 47(1): 19-23.

DOI：10.1080/00387010.2013.775464

• electrospray ionization
• polyphenolic glycosides
• regioisomeric O-acylated monosaccharide
• structural differentiation
• tandem mass spectrometry

Isomeric polyphenolic glycosides that possess regioisomeric O-acylated saccharide residues can be found in many medicinal plants and their preparations as well as some synthetic intermediates. The need for a pure and sufficient quantity of compounds for nuclear magnetic resonance measurements calls for a microanalytical method for their rapid differ entiation. In the current investigation, an electrospray ionization-tandem mass spectrometric method was developed for rapid differentiation of two pairs of regioisomeric acylated monosaccharides, quercetin-3-O-(3-O-galloyl)--L-rhamnopyranoside and quercetin-3-O-(2-O-galloyl)--L-rhamnopyranoside as well as quercetin-3-O-(3-O-galloyl)--D-glucopyranoside and quercetin-3-O-(2-O-galloyl)--D-glucopyranoside; the mechanisms governing their similar or differential fragmentations are also discussed. The work may facilitate the structural determination of these regioisomers and their analogs in pure form or in complex matrix.

• 出版日期2014-1-2
• 单位贵州医科大学