Pyrene-based water dispersible orange emitter for one- and two-photon fluorescence cellular imaging

作者：Wang Guan; Geng Junlong; Zhang Xinhai; Cai Liping; Ding Dan; Li Kai; Wang Long; Lai Yee Hing^*; Liu Bin

来源：Polymer Chemistry, 2012, 3(9): 2464-2470.

DOI：10.1039/c2py20271a

A water-dispersible orange emitter (Pyrene4BTF-PEG-TAT) containing pyrene as the donor core and benzothiadiazole as the acceptor is synthesized through click chemistry for one-and two-photon fluorescence imaging application. Pyrene4BTF-PEG-TAT has an emission maximum at 586 nm in water with a large Stokes shift of 181 nm. It self-assembles into nanoparticles with diameters of similar to 190 nm in water. The nanoparticles have a fluorescence quantum yield of 0.11 and a two-photon absorption (TPA) cross-section of 512 GM at 820 nm based on molecule. Pyrene4BTF-PEG-TAT could be effectively internalized by HeLa cells to show bright red fluorescence upon both one-and two-photon excitation. The successful demonstration of the first water-dispersible pyrene-based TPA material will trigger more research on developing red emitters for bioimaging applications.

• 出版日期2012