A Quiescent, Regeneration-Responsive Tissue Engineered Mesenchymal Stem Cell Bone Marrow Niche Model via Magnetic Levitation

作者：Lewis Emily Elizabeth Louise; Wheadon Helen; Lewis Natasha; Yang Jingli; Mulling Margaret; Hursthouse Andrew; Stirling David; Dalby Matthew John; Berry Catherine Cecilia^*

来源：ACS Nano, 2016, 10(9): 8346-8354.

DOI：10.1021/acsnano.6b02841

• mesenchymal stem cells
• bone marrow niche
• magnetic nanoparticles
• tissue engineering
• migration
• scratch assay
• wound homing
• differentiation

The bone marrow niche represents a specialized environment that regulates mesenchymal stem cell quiescence and self-renewal, yet fosters stem cell migration and differentiation upon demand. An in vitro model that embodies these features would open up the ability to perform detailed study of stem cell behavior. In this paper we present a simple bone marrow-like niche model, which comprises of nanomagnetically levitated stem cells cultured as multicellular spheroids within a type I collagen gel. The stem cells maintained are nestin positive and remain quiescent until regenerative demand is placed upon them. In response to coculture wounding, they migrate and appropriately differentiate upon engraftment. This tissue engineered regeneration-responsive bone marrow-like niche model will allow for greater understanding of stem cell response to injury and also facilitate as a testing platform for drug candidates in a multiwell plate format.

• 出版日期2016-9