科研之友
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摘要
Purpose: Reduced or delayed neovascularization is a major obstacle with regard to tissue-engineered constructs. The aim of this study was to evaluate the early microvascular response to a novel degradable epsilon-caprolactone terpolymer matrix.
Methods: epsilon-caprolactone terpolymer matrices (Suprathel Plus (R); Institute of Textile and Process Engineering, Denkendorf, Germany) were implanted into dorsal skinfold chambers of balb/c mice (n=10). Microcirculatory changes were observed by intravital fluorescence microscopy. Scaffolding matrices from PEGT/PBT copolymer were used as controls (n=10).
Results: The formation of de novo vascular networks within both scaffolding matrices was noted throughout the experiment. A vascular ingrowth of perfused microvessels into the matrices up to 600 mu m apart from the edge was noted within 10 days of implantation. The earliest signs of neoangiogenesis were visible in epsilon-caprolactone terpolymer matrices on day 1. In both scaffolds the new developed vessels extended centripetally from the border of the matrices towards the center and anastomosed to form a perfused microvascular network. There was significantly earlier onset of vascularization, increased vascularized area and higher vessel density in epsilon-caprolactone terpolymer matrices compared to PEGT/PBT copolymer matrices were observed.
Conclusions: The scaffolding matrix from epsilon-caprolactone terpolymer allowed for an earlier and more intense induction of angiogenesis and displayed the tendency to vascularize more rapidly within a shorter period of time after transplantation compared to PEGT/PBT copolymer scaffolds, thus indicating its potential application for tissue engineering purposes.
- 出版日期2010-12
