Monitoring Campylobacter in the poultry production chain - From culture to genes and beyond

作者:Josefsen Mathilde H*; Bhunia Arun K; Engvall Eva Olsson; Fachmann Mette S R; Hoorfar Jeffrey
来源:Journal of Microbiological Methods, 2015, 112: 118-125.
DOI:10.1016/j.mimet.2015.03.007

摘要

Improved monitoring tools are important for the control of Campylobacter bacteria in poultry production. Standardized reference culture methods issued by national and international standardization organizations are time-consuming, cumbersome and not amenable to automation for screening of large numbers of samples. The ultimate goal for rapid monitoring of Campylobacter is to prevent contaminated meat from entering the food market. Currently, real-time PCR is fulfilling abovementioned criteria to a certain extent. Further development of real-time PCR, microarray PCR, miniaturized biosensors, chromatographic techniques and DNA sequencing can improve our monitoring capacity at a lower cost. Combined with innovative sampling and sample treatment, these techniques could become realistic options for on-farm and liquid-sample monitoring at slaughterhouses.

  • 出版日期2015-5