Airway epithelial NF-B activation promotes the ability to overcome inhalational antigen tolerance

作者：Ather J L; Foley K L; Suratt B T; Boyson J E; Poynter M E^*

来源：Clinical and Experimental Allergy, 2015, 45(7): 1245-1258.

DOI：10.1111/cea.12491

• allergic airway disease
• dendritic cells
• inhalational antigen tolerance
• NF-kappa B
• T regulatory cells

BackgroundInhalational antigen tolerance typically protects against the development of allergic airway disease but may be overcome to induce allergic sensitization preceding the development of asthma.
ObjectivesWe examined in vivo whether pre-existing inhalational antigen tolerance could be overcome by activation of the transcription factor NF-B in conducting airway epithelial cells, and used a combination of in vivo and in vitro approaches to examine the mechanisms involved.
MethodsWild-type and transgenic mice capable of expressing constitutively active IB kinase (CAIKK) in airway epithelium were tolerized to inhaled ovalbumin. Twenty-eight days later, the transgene was transiently expressed and mice were exposed to inhaled OVA on Day 30 in an attempt to overcome inhalational tolerance.
ResultsFollowing ovalbumin challenge on days 40-42, CAIKK mice in which the transgene had been activated exhibited characteristic features of allergic airway disease, including airway eosinophilia and methacholine hyper-responsiveness. Increases in the CD103(+) and CD11b(HI) lung dendritic cell populations were present in CAIKK mice on Day 31. Bronchoalveolar lavage from mice expressing CAIKK mice induced CD4(+) T cells to secrete T(H)2 and T(H)17 cytokines, an effect that required IL-4 and IL-1 signalling, respectively. CAIKK mice on Dox demonstrated increased numbers of innate lymphoid type 2 cells (ILC2) in the lung, which also exhibited elevated mRNA expression of the T(H)2-polarizing cytokine IL-4. Finally, airway epithelial NF-kB activation induced allergic sensitization in CAIKK mice on Dox that required IL-4 and IL-1 signalling in vivo.
ConclusionsOur studies demonstrate that soluble mediators generated in response to airway epithelial NF-B activation orchestrate the breaking of inhalational tolerance and allergic antigen sensitization through the effects of soluble mediators, including IL-1 and IL-4, on pulmonary dendritic cells as well as innate lymphoid and CD4(+) T cells.

• 出版日期2015-7