Zwitterionic stealth peptide-protected gold nanoparticles enable long circulation without the accelerated blood clearance phenomenon

作者：Zhao, Jue; Qin, Zhihui; Wu, Jina; Li, Lijie^*; Jin, Qiao^*; Ji, Jian

来源：Biomaterials Science, 2017, 6(1): 200-206.

DOI：10.1039/c7bm00747g

• DRUG-DELIVERY
• SURFACE MODIFICATION
• PEGYLATED LIPOSOMES
• REPEATED INJECTIONS
• CATIONIC LIPOSOMES
• NANOCARRIERS
• ACCUMULATION
• THERAPY
• ACID
• POLYPHOSPHOESTER

Poly(ethylene glycol) (PEG), which is considered as a gold standard for surface modification of nano-particles in biomedical applications, has been reported to encounter the accelerated blood clearance (ABC) phenomenon after repeated administration. Herein, as an ideal substitute for PEG, a zwitterionic peptide sequence of alternating negatively charged glutamic acid (E) and positively charged lysine (K) was designed as a good candidate for surface modification of nanoparticles without the ABC phenomenon in vivo. PEG-protected gold nanoparticles (AuNP-PEG) suffered from a serious ABC phenomenon with very fast blood clearance after repeated injection. Meanwhile, the plasma IgM and IgG levels were significantly increased after the repeated injection of AuNP-PEG. However, zwitterionic stealth peptide-protected gold nanoparticles (AuNP-EK10) could avoid the activation of the ABC phenomenon. The increase of IgM and IgG levels was not observed after the repeated injection of AuNP-EK10. More interestingly, compared to AuNP- PEG, more AuNP-EK10 could be accumulated in tumor tissues after repeated injection of the nanoparticles to tumor-bearing nude mice, which might be especially important for the design of drug nanocarriers in cancer therapy.

• 出版日期2017-1-1
• 单位浙江大学; 浙江省人民医院