An Optimized Sensor Array Identifies All Natural Amino Acids

作者：Wang Benhua; Han Jinsong; Bojanowski N Maximilian; Bender Markus; Ma Chao; Seehafer Kai; Herrmann Andreas; Bunz Uwe H F^*

来源：ACS Sensors, 2018, 3(8): 1562-1568.

DOI：10.1021/acssensors.8b00371

• sensor array
• amino acid
• poly(para-phenyleneethynylene)s
• cucurbiturils
• acridine orange
• green fluorescent protein

Wet-chemical discrimination of amino acids is still a challenge due to their structural similarity. Here, an optimized self-assembled eight-member sensor array is reported. The optimized sensor array stems from the combination of elements of different tongues, containing poly(para-phenyleneethynylene)s (PPE) and a supercharged green fluorescent protein (GFP) variant. The responsivity of the sensor dyes (PPEs and GFP) is enhanced in elements that contain adjuvants, such as metal salts but also cucurbit[7]uril (CB[7]) and acridine orange; a suitable and robust eight element array discriminates all of the 20 natural amino acids in water at 25 mM concentration with 100% accuracy. The results group well to the amino acid type, i.e., hydrophobic, polar, and aromatic ones.

• 出版日期2018-8