A sensitive immunoassay for parathion based on covalent linkage between small molecules hapten microtiter plates surface

作者：Sai, Na; Sun, Wenjing; Wu, Yuntang; Sun, Zhong; Huang, Guowei^*

来源：Journal of the Iranian Chemical Society, 2017, 14(1): 257-268.

DOI：10.1007/s13738-016-1009-5

• Competive inhibition enzyme-linked immunosorbent assay
• Covalent linkage
• Parathion
• Small molecule hapten

A sensitive competitive inhibition enzyme-linked immunosorbent assay (CIELISA) for the detection of parathion has been developed. In this assay, a small molecule hapten II (O,O-diethyl O-4-aminophenyl phosphorothioate) was covalent linked to glutaric dialdehyde treated-microtiter plates. In addition, 4-(ethoxy(4-nitrophenoxy) phosphorothioylamino) butanoic acid-ovalbumin (hapten I-OVA) conjugate served as the coating antigen for comparison with directly hapten II covalent linked plates in the CIELISA format. The developed assay demonstrated highly sensitivity (IC10 was 0.08 ng mL(-1)) selectivity and stability. In samples analysis, the results of parathion detected by this assay were in accordance with which obtained by high-performance liquid chromatography.

• 出版日期2017-1
• 单位天津医科大学