Development of Direct Competitive ELISA for Residue Analysis of Fungicide Chlorothalonil in Vegetables

作者：Okazaki Fumiko; Hirakawa Yuki; Yamaguchi Murakami Yukie; Harada Ayako; Watanabe Eiki; Iwasa Seiji; Narita Hiroshi; Miyake Shiro^*

来源：Journal of the Food Hygienic Society of Japan, 2014, 55(2): 65-72.

DOI：10.3358/shokueishi.55.65

• monoclonal antibody
• immunoassay
• dc-ELISA
• chlorothalonil

A direct competitive (dc)-ELISA was developed for rapid and simple determination of chlorothalonil residue in vegetables. A carboxylic acid derivative of pentachlorophenol was used to prepare an anti-chlorothalonil monoclonal antibody (MoAb) that showed adequate reactivity for dc-ELISA. Before homogenization of vegetable samples, phosphoric acid was added (vegetable-10% phosphoric acid (2: 1, w/v)) to block enzymatic decomposition of chlorothalonil. The use of phosphate buffer (100 mmol/L, pH 7.0) minimized the influence of phosphoric acid on competitive reaction in the dc-ELISA. Working range was 0.10 to 6.0 ng/mL in the optimized dc-ELISA. The recovery of chlorothalonil spiked in cucumber and eggplant was 97.1 to 125%. The results correlated well with those obtained by HPLC analysis. The dc-ELISA could rapidly determine chlorothalonil after a simple sample preparation procedure.

• 出版日期2014-4