Analysis of Phosphate in Rhizosphere Soil Solution Samples Using Capillary Electrophoresis

作者：Wang Zhen Yu; Wen Sheng Fang; Li Ai Feng^*

来源：Chinese Journal of Analytical Chemistry, 2010, 38(1): 87-90.

• Phosphate
• Soil solution
• Capillary electrophoresis

Analytical method of capillary zone electrophoresis with indirect UV detection was optimized and validated to determine the phosphate in rhizosphere soil solution samples. The effect of detection wavelength, electrolyte composition, separation voltage and temperature were investigated. Analyses were performed in a 56 cm uncoated fused-silica capillary(length to the detector window) with reversed electroosmotic flow, anodic detection in 205 nm, separation voltage - 20 kV, and temperature 25 degrees C. The electrolyte contained 32 mmol/L tris-hydroxymethyl-aminomethane, 4 mmol/L 1, 2, 4-benzene-tricarboxylic trimellitic acid and 0.3 mmol/L, tetradecyl-trimethylammonium bromide, pH 8.5. Under the conditions, the negative effect of the inorganic anion, such as Cl(-), SO(4)(2-) and NO(3)(-), can be reduced on phosphate. The detection limit was 0.68 mg/L(S/N = 3) and the recovery was 87.2% - 99.4%. The method has been applied to the determination of the concentration of phosphate in the rhizoshere soil solution.

• 出版日期2010-1
• 单位中国海洋大学