Multiple resistance to ACCase and AHAS-inhibiting herbicides in shortawn foxtail (Alopecurus aequalis Sobol.) from China

作者：Guo, Wenlei; Yuan, Guohui; Liu, Weitang; Bi, Yaling; Du, Long; Zhang, Chao; Li, Qi; Wang, Jinxin^*

来源：Pesticide Biochemistry and Physiology, 2015, 124: 66-72.

DOI：10.1016/j.pestbp.2015.04.006

• Alopecurus aequalis
• ACCase gene
• AHAS gene
• Multiple resistance
• Mutation

Shortawn foxtail (Alopecurus aequalis) is a troublesome grass weed infesting winter wheat and oilseed rape productions in China. Fenoxaprop-p-ethyl and mesosulfuron-methyl failed to control shortawn foxtail of AHSX-1 population collected from a wheat field in Shou County, Anhui province. Molecular analyses revealed that Asp(2078)Gly mutation of ACCase and Trp(574)Leu mutation of AHAS were present in plants of the AHSX-1 population. The homozygous plants were isolated and cultured until seed maturity. Whole-plant herbicide bioassays were conducted in the greenhouse using the purified seeds of F1 generation. Dose-response experiments showed that the AHSX-1 population has evolved a very high level resistance to fenoxaprop-p-ethyl (RI = 275) and mesosulfuron-methyl (RI = 788). To determine the sensitivity to other herbicides, assays were conducted at the single recommended rate of each herbicide. Based on the results, the AHSX-1 population was considered to be highly resistant to clodinafop-propargyl, pyroxsulam and flucarbazone-sodium, moderately or highly resistant to quizalofop-p-ethyl, clethodim, sethoxydim and pinoxaden, and susceptible to isoproturon and chlorotoluron. This is the first report of Asp(2078)Gly mutation in shortawn foxtail and the two robust dCAPS markers designed could quickly detect Asp(2078) and Trp(574) mutations in ACCase and AHAS gene of shortawn foxtail, respectively.

• 出版日期2015-10
• 单位山东农业大学; 安徽科技学院