A Monoclonal Antibody SZ-117 That Recognizes Filamin A Derived from Tumor Cells

作者：Bu, Zibin; Liu, Ruifang; Shang, Bingxue; Cao, Zhifei; Pan, Yanyan; Zhou, Quansheng^*; Ruan, Changgeng

来源：Hybridoma, 2012, 31(3): 214-218.

DOI：10.1089/hyb.2011.0109

SZ117 is a monoclonal antibody against matrix metalloproteinase-2 (MMP-2) and exhibits anti-tumor angiogenic effect. In this study, we observed that SZ117 bound to a 280 kDa protein, which was detected in tumor cell-derived Matrigel and various tumor cells. Using immunoprecipitation, mass spectrometry analysis, and Western blot analysis, we identified the 280 kDa protein as filamin A and found that filamin A and its degraded products, notably a 53 kDa fragment, were released from a variety of tumor cells. This suggests that SZ117 is useful in the study of the pathogenesis of filamin A and that blockage of filamin A by SZ117 might contribute to the antitumor angiogenic effect of the monoclonal antibody.

• 出版日期2012-6
• 单位苏州大学