Probing single molecule interactions by AFM using bio-functionalized dendritips

作者：Jauvert Eric; Dague Etienne; Severac Marjorie; Ressier Laurence; Caminade Anne Marie; Majoral Jean Pierre; Trevisiol Emmanuelle^*

来源：Sensors and Actuators B: Chemical , 2012, 168: 436-441.

DOI：10.1016/j.snb.2012.04.048

• AFM tip functionalization
• Phosphorus dendrimers
• Single molecule force spectroscopy (SMFS)
• Molecular recognition
• Antigen/antibody interaction

A new strategy for AFM (atomic force microscopy) tip functionalization based on the use of aldehyde-phosphorus dendrimers for the immobilization of biomolecules such as proteins (e.g. antibodies) is presented. Firstly functionalized with amino groups, the tips are reacted with dendrimers leading to dendrimer-activated tips (so-called dendritips). Free aldehyde functions on the dendrimer are therefore available to react with amino-functions present on every protein and many biomolecules. Using biofunctionalized-dendritip, single molecule force interactions between glutathione-S-transferase (GST) and its cognate antibody immobilized on dendritips (67 +/- 11 pN for single interaction) were probed by AFM spectroscopy. The specificity of our measurements was demonstrated by performing blocking tests resulting in the loss of interactions.

• 出版日期2012-6-20