Development and validation of ultra high performance-liquid chromatography-tandem mass spectrometry based methods for the determination of neonicotinoid insecticides in honey

作者：Valverde Silvia; Ibanez Maria; Bernal Jose L^*; Nozal Maria J; Hernandez Felix; Bernal Jose^*

来源：Food Chemistry, 2018, 266: 215-222.

DOI：10.1016/j.foodchem.2018.06.004

• Honey
• Mass spectrometry
• Neonicotinoid insecticides
• QuEChERS
• Solid phase extraction
• Ultra-high performance liquid chromatography

In this study, the feasibility of two sample treatments has been evaluated for the determination of seven neonicotinoid insecticides in honey from different botanical origins using ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS). A solid phase extraction with a polymeric sorbent (Strata((R)) X) is proposed for analyzing dark honeys, while a QuEChERS (quick, easy, cheap, effective, rugged and safe) approach is recommended for light honeys. Chromatographic analysis (6 min) was performed on a core-shell column (Kinetex((R)) EVO C-18). The proposed methods were fully validated using two different MS/MS systems: quadrupole-time-of-flight and triple quadrupole. The results showed that the best overall analytical performance was achieved using triple quadrupole, mainly due to its better sensitivity and the reduced influence of the matrix onto the analyte signals. The methods developed were applied to the analysis of commercial honey samples from different regions of Spain, as well as from experimental apiaries.

• 出版日期2018-11-15