Allopurinol preconditioning attenuates renal ischemia/reperfusion injury by inhibiting HMGB1 expression in a rat model

作者：Zhou, Jiang-qiao; Qiu, Tao^*; Zhang, Lu; Chen, Zhong-bao; Wang, Zhi-shun; Ma, Xiao-xiong; Li, Dongyu

来源：Acta Cirurgica Brasileira, 2016, 31(3): 176-182.

DOI：10.1590/S0102-865020160030000005

• Inflammation
• Ischemia
• Reperfusion
• Allopurinol
• Apoptosis
• Rats

PURPOSE: To investigate the potential effects of pretreatment with allopurinol on renal ischemia/reperfusion injury (IRI) in a rat model.
METHODS: Twenty four rats were subjected to right kidney uninephrectomy were randomly distributed into the following three groups (n=8): Group A (sham-operated group); Group B (ischemic group) with 30 min of renal ischemia after surgery; and Group C (allopurinol + ischemia group) pretreated with allopurinol at 50 mg/kg for 14 days. At 72 h after renal reperfusion, the kidney was harvested to assess inflammation and apoptosis.
RESULTS: Pretreatment with allopurinol significantly improved renal functional and histological grade scores following I/R injury (p< 0.05). Compared with Group B, the expression levels of caspase-3 and Bax were markedly reduced in Group C, meanwhile, whereas expression of bcl-2 was clearly increased (p< 0.05). A newly described marker of inflammation, High Mobility Group Box 1(HMGB1), showed reduced expression in Group C (p< 0.05).
CONCLUSION: Pretreatment with allopurinol had a protective effect on kidney ischemia/reperfusion injury, which might be related to the inhibition of HMGB1 expression.

• 出版日期2016-3
• 单位武汉大学