Measurement of serum remnant-like lipoprotein particle-triglyceride (RLP-TG) and RLP-TG/total TG ratio using highly sensitive triglyceride assay reagent

作者：Nakano Takamitsu; Tokita Yoshiharu; Nagamine Takeaki; Tanaka Akira; Okazaki Mitsuyo; Yatsuzuka Shinichi; Tamei Noriko; Shimomura Younosuke; Ando Yoshitaka; Akamatsu Suguru; Fujita Masaki Q; Nakajima Katsuyuki^*

来源：Clinica Chimica Acta, 2011, 412(1-2): 71-78.

DOI：10.1016/j.cca.2010.09.040

• Remnant-like lipoprotein particles (RLP)
• RLP-triglyceride (RLP-TG)
• RLP-cholesterol (RLP-C)
• Postprandial hyperlipidemia
• RLP-TG/total TG ratio
• Metabolic syndrome

Background: Serum concentration of remnant-like lipoprotein particles (RLP) have been measured by cholesterol as RLP-C for clinical diagnostic purpose. However, the measurement of TG in RLP and the ratio of RLP-TG/total TG has not been well established.
Method: Highly sensitive triglyceride assay reagent (TG-EX) was used for RLP-TG assay and compared with the previously used TG reagent (Determiner LTGII). Sera in health check-up populations, cardiovascular disease, diabetes and oral fat load cases were used for the evaluation of the new RLP-TG assay. Serum TC, TG, HDL-C, LDL-C and RLP-C concentrations were also determined in above cases.
Results: The detection limit of new RLP-TG using TG-EX was 2.0 mg/dl. The within-run imprecision (n = 10) was CV = 3.0% (RLP-TG: 4.1 mg +/- 0.7 mg/dl), CV = 1.4% (RLP-TG: 42.0 +/- 0.6 mg/dl) and CV = 0.5% (RLP-TG: 100.6 +/- 0.6 mg/dl). Cut-off value (75 percentile) of RLP-TG determined in the fasting Japanese population was 13.1 mg/dl in men and 9.9 mg/dl in women. In patients with metabolic syndrome, cardiovascular disease and diabetes. RLP-TG levels were significantly higher than those in normal control subjects. RLP-TG levels increased significantly after an oral fat load and the ratio of RLP-TG/total TG increased>3-fold compared to the ratio in the fasting state. Approximately 80% of TG increased after an oral fat load was TG derived from remnant lipoproteins.
Conclusion: Normal range of plasma RLP-TG in the fasting Japanese population was first determined using a highly sensitive TG assay reagent. RLP-TG was shown to be higher in cases with metabolic syndrome, cardiovascular disease. etc and a better marker than RLP-C for the measurement of postprandial remnant lipoproteins, together with total TG for RLP-TG/total TG ratio.

• 出版日期2011-1-14